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For practitioners · 49 one-page protocols

Protocols

One-page references for each method: dosing, schedules, equipment and precautions. Grouped by how each method is delivered, and each one links to its full article.

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Which protocol fits?

At homeGas, oil, glycerin and water methods with a small generator or none.Gas methodsOzone oilGlycerinWater
In a clinicBlood, IV and organ methods for licensed practitioners.IV, EBOO and moreSkin
InjectionsJoint, spine, trigger point, acupuncture point, PRP and prostate.12 injection sheets
DentistryDecay, gums, root canal, oral surgery and mouth sores.6 dental sheets
VeterinaryDogs, cats, horses and cattle.3 veterinary sheets

For licensed practitioners. These are educational references only. Follow your scope of practice and local regulations, and screen every patient for contraindications. Using ozone at home? Start with the home guide.

Start here

Gas methods

6 protocols · Home or clinic · insufflation, bagging and sauna.

Ozone and oxygen by rectum, ear, vagina, skin (bagging, cupping, sauna) or breathing oil. Most can be done at home with a small generator; new users usually start with rectal.

Home or clinic

Rectal Ozone Insufflation Protocol

Dosing, schedule, equipment and precautions for rectal ozone insufflation, including a slower protocol for sensitive patients.

Home or clinic

Ozone Ear Insufflation Protocol

Dosing, equipment, helpful hints and precautions for ozone ear insufflation.

Home or clinic

Vaginal Ozone Insufflation Protocol

Dosing, schedule and precautions for vaginal ozone insufflation.

Home or clinic

Ozone Limb Bagging and Cupping Protocol

Concentration, weekly step-down schedule, helpful hints and precautions for ozone limb bagging and cupping.

Home or clinic

Breathing Ozonated Oil (BOO) Protocol

Concentration, flow, schedule and precautions for breathing the vapor from ozone bubbled through olive oil.

Home or clinic

Ozone Sauna Protocol

Concentration, session length, temperature and safety for the ozone sauna, with the head kept outside the chamber.

Patients doing this at home? Send them the free guide.Free home guide →

Ozone oil

6 protocols · At home · ozone carried in oil, to use on skin, in the mouth or by capsule.

Ozone carried in oil for skin, mouth, capsules and suppositories. No generator is needed to use it, and one sheet explains why we don't recommend making it at home.

Home or clinic

Topical Ozonated Oil and Cream for Skin Protocol

Strength, how often to apply, and precautions for ozonated oil and cream used on the skin.

Home

Ozone Oil Pulling Protocol

How much ozonated oil to swish, for how long and how often, with precautions and what the oral-health studies used.

Home

Ozone Oil Suppositories Protocol

How many ozonated-oil suppositories to use, how often, rectal and vaginal differences, and precautions.

Home

Ozone Oil Capsules Protocol

Dose, timing and cautions for ozonated-oil capsules taken by mouth, with a plain note on the thin evidence.

Home

Ozone Oil Massage Protocol

How much ozonated oil to use for muscle and joint massage, how often, and precautions, with the thin evidence stated plainly.

Home

Making Ozonated Oil Protocol (not advised)

How manufacturers make ozonated oil, with the Madrid caution. MediSkill does not recommend making it at home.

Patients doing this at home? Send them the free guide.Free home guide →

Ozonated glycerin

6 protocols · At home · no generator needed.

A water-friendly alternative to ozone oil for nebulizing, drinking, skin, nose and mouth. Never for IV or injection.

No generator

Ozonated Glycerin Protocol

Uses, mixing strengths, nebulizer set-up and precautions for ozonated glycerin, which needs no generator.

Home

Nebulizing Ozonated Glycerin Protocol

Strength, amount, mixing table and a step-by-step session for nebulizing diluted ozonated glycerin at home.

Home

Drinking Ozonated Glycerin Protocol

How much ozonated glycerin to stir into a drink, how to build up over the first weeks, and what to watch for.

Home

Ozonated Glycerin for Skin and Beauty Protocol

Mixing strengths, how often to use ozonated glycerin on the skin, and how to start gently.

Home

Ozonated Glycerin Nasal Spray Protocol

How to mix ozonated glycerin with saline for a nasal pump spray, how much to spray, and how often.

Home

Ozonated Glycerin Mouthwash and Gum Protocol

How to mix ozonated glycerin into a mouthwash, how long to swish, and how to use a small amount on the gums.

Patients doing this at home? Send them the free guide.Free home guide →

Water

1 protocol · Home or clinic · water bubbled with ozone.

Water bubbled with ozone, used to drink, rinse or bathe. One sheet covers making it and using it.

Home or clinic

Ozone-Infused Water Protocol

Concentration, bubbling time, amounts and precautions for making and using ozone-infused water.

Patients doing this at home? Send them the free guide.Free home guide →

Skin

3 protocols · Skin conditions · home steps.

Acne and rosacea, eczema and psoriasis, and fungal infections, with the steps you can do at home.

Home

Acne and Rosacea Ozone Protocol

Home ozone steps for acne and rosacea: an ozonated water wash and ozonated oil, with what the studies used.

Home

Atopic Dermatitis and Psoriasis Ozone Protocol

Home ozone for atopic dermatitis and psoriasis: ozonated oil and ozone water, with what the studies used.

Home

Fungal Skin and Nail Infection Ozone Protocol

Oil and water steps for ringworm, athlete's foot, candida and nail fungus, with the scalp spray and oil.

In the clinic

6 protocols · Licensed practitioners only · blood, IV and organ methods.

Blood, IV and organ methods for licensed practitioners: autohemotherapy, EBOO, and bladder instillation, plus the saline drip and 10-Pass methods we do not advise.

Clinic

IV Ozone Protocol: Major Autohemotherapy (MaH)

Equipment, step-by-step directions, dosing and titration for IV ozone by major autohemotherapy (MaH).

Clinic

Minor Autohemotherapy (MiAH) Protocol

Concentration levels, steps, frequency and precautions for minor autohemotherapy (MiAH).

Practitioner

EBOO (cautioned)

Madrid's ozone concentration, gas volume, blood flow and equipment notes for extracorporeal blood oxygenation-ozonation, an advanced hospital procedure. Standard IV ozone (MAH) comes first.

Clinic

Bladder (Vesicourethral) Ozone Instillation Protocol

Preparation, concentration and precautions for bladder irrigation with ozonized water, performed by a clinician.

Clinic

10-Pass Ozone Therapy Protocol

How 10-Pass (high-dose multistep IV ozone) is commonly done, with the Madrid Declaration's position on it. MediSkill does not advise it.

Clinic

Ozonated Saline IV Drip (O3SS) Protocol

Weight-based dosing, preparation, concentration calculation and safety for the ozonated saline (O3SS) IV drip.

New to ozone? Start with the free guide.Get the free guide →

Injections (practitioner)

12 protocols · Licensed practitioners only · joint, spine and point injections.

Joint, spine, point and prostate injections for practitioners, with gas concentration, volume, sites and spacing for each.

Clinic

Knee Ozone Injection Protocol

Landmarks, injection angle, gas volume and schedule for ozone knee injections, with the Prolozone mixture and ozone-oxygen approaches side by side.

Clinic

Hip Ozone Injection Protocol

Lateral approach, landmarks, needle and schedule for ozone hip injections, with the Prolozone mixture and ozone-oxygen approaches side by side.

Clinic

Shoulder Ozone Injection Protocol

Posterior landmarks, fluid volume and schedule for ozone shoulder injections, including a reported septic arthritis case, with Prolozone and ozone-oxygen approaches side by side.

Clinic

Elbow Ozone Injection Protocol

Fluid and gas volumes and schedule for ozone elbow injections, with the Prolozone mixture and ozone-oxygen approaches side by side.

Clinic

Ankle Ozone Injection Protocol

Superficial injection around the ankle's stabilizing structures, nerve cautions and schedule, with the Prolozone mixture and ozone-oxygen approaches side by side.

Clinic

Neck Ozone Injection Protocol

Cervical landmarks, levels to avoid and the needle for ozone neck injections, with the Prolozone mixture and ozone-oxygen approaches side by side.

Clinic

Back Ozone Injection Protocol

Lumbar landmarks, injection sites and volumes for paravertebral ozone back injections, with the Prolozone mixture and ozone-oxygen approaches side by side.

Practitioner · Injection

Trigger Point Ozone Injection Protocol

Concentration, volume per point, points per session and schedule for ozone trigger point injections, with Madrid and study doses side by side.

Practitioner · Injection

Acupuncture Point Ozone Injection Protocol

Microdose volume, concentration and technique for ozone injected into acupuncture points, with Madrid and case series doses side by side.

Practitioner · Injection

Subcutaneous Ozone Injection Protocol

Concentration, volume per site, session limits and the glove technique for subcutaneous ozone injection, from Madrid and ISCO3.

Practitioner · Injection

PRP and Ozone Injection Protocol

Order of injection, ozone concentration and volume, and PRP preparation from the studies that combined ozone with platelet-rich plasma.

Practitioner · Injection

Prostate Ozone Injection Protocol

Madrid's gas volume, concentration and weekly schedule for ozone injections in bacterial prostatitis and benign prostatic hyperplasia, with what small studies reported.

Dentistry (practitioner)

6 protocols · Dental professionals only · gas in the mouth and jaw.

Ozone gas, water and oil for decay, gums and implants, oral surgery, root canals, mouth sores and jaw osteonecrosis. Written for dental professionals.

Dentistry

Dental Ozone Protocol: Tooth Decay and Sensitivity

Settings published human studies used for gas on tooth decay, dentin hypersensitivity, bleaching sensitivity and white-spot prevention, by device family.

Dentistry

Dental Ozone Protocol: Gum Disease, Peri-Implantitis and Implant Stability

Settings published human studies used for gas in gum pockets, around inflamed implants and at implant placement. As an add-on to scaling, about half the trials found no added benefit.

Dentistry

Dental Ozone Protocol: Oral Surgery and Healing

Settings published human studies used for gas after tooth extraction, at the implant drill site, on gum graft wounds and for orthodontic pain.

Dentistry

Dental Ozone Protocol: Root Canal Disinfection and Pulp Capping

Settings published reports used for gas in root canals and on the exposed pulp. The evidence is case reports and one small controlled study.

Dentistry

Dental Ozone Protocol: Mouth Sores, Lichen Planus and Ulcers

Settings published human studies used for gas on aphthous ulcers, oral lichen planus and chemotherapy or post-extraction ulcers.

Dentistry

Dental Ozone Protocol: Medication-Related Osteonecrosis of the Jaw (MRONJ)

Settings published reports used for gas in medication-related osteonecrosis of the jaw. Treatment reports are uncontrolled; the one prevention RCT found no difference.

Veterinary

3 protocols · Veterinary use · dogs, cats, horses and cattle.

Ozone for dogs, cats, horses and cattle, including common horse conditions. Doses scale with species and body weight.

Veterinary

Veterinary Ozone Protocol: Dogs and Cats

Routes, concentrations, volumes and schedules Madrid gives for ozone in dogs and cats: blood, rectal, injection, bagging, ozonated water and oil.

Veterinary

Veterinary Ozone Protocol: Horses and Cattle

Systemic and local routes Madrid gives for horses and cows: autohemotherapy, rectal and vaginal insufflation, joint and spine injections, bagging, ozonated water, oil and mastitis.

Veterinary

Veterinary Ozone Protocol: Horse Conditions

What Madrid lists for hooves, joints, tendons, back, skin, foals, wounds, reproduction and performance in horses, with concentrations, volumes and schedules.

In their own words

Lindsay O'Shea, APRN

Functional medicine clinic founder · attended a live event

“Not just the science behind ozone therapy… but also how do you take this and implement it into practice immediately.”

Dr. Akiba Green

Integrative wellness clinic, Lake Norman, North Carolina

“It’s the best decision I’ve done in the last six years.”

Next step

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The Definitive Guide to Getting Started with Ozone Therapy: the methods, the equipment and staying safe.

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