For practitioners · 49 one-page protocols
One-page references for each method: dosing, schedules, equipment and precautions. Grouped by how each method is delivered, and each one links to its full article.
Want the background first? See How to do it.
Which protocol fits?
For licensed practitioners. These are educational references only. Follow your scope of practice and local regulations, and screen every patient for contraindications. Using ozone at home? Start with the home guide.
Start here
6 protocols · Home or clinic · insufflation, bagging and sauna.
Ozone and oxygen by rectum, ear, vagina, skin (bagging, cupping, sauna) or breathing oil. Most can be done at home with a small generator; new users usually start with rectal.
Home or clinic
Rectal Ozone Insufflation ProtocolDosing, schedule, equipment and precautions for rectal ozone insufflation, including a slower protocol for sensitive patients.
Home or clinic
Ozone Ear Insufflation ProtocolDosing, equipment, helpful hints and precautions for ozone ear insufflation.
Home or clinic
Vaginal Ozone Insufflation ProtocolDosing, schedule and precautions for vaginal ozone insufflation.
Home or clinic
Ozone Limb Bagging and Cupping ProtocolConcentration, weekly step-down schedule, helpful hints and precautions for ozone limb bagging and cupping.
Home or clinic
Breathing Ozonated Oil (BOO) ProtocolConcentration, flow, schedule and precautions for breathing the vapor from ozone bubbled through olive oil.
Home or clinic
Ozone Sauna ProtocolConcentration, session length, temperature and safety for the ozone sauna, with the head kept outside the chamber.
6 protocols · At home · ozone carried in oil, to use on skin, in the mouth or by capsule.
Ozone carried in oil for skin, mouth, capsules and suppositories. No generator is needed to use it, and one sheet explains why we don't recommend making it at home.
Home or clinic
Topical Ozonated Oil and Cream for Skin ProtocolStrength, how often to apply, and precautions for ozonated oil and cream used on the skin.
Home
Ozone Oil Pulling ProtocolHow much ozonated oil to swish, for how long and how often, with precautions and what the oral-health studies used.
Home
Ozone Oil Suppositories ProtocolHow many ozonated-oil suppositories to use, how often, rectal and vaginal differences, and precautions.
Home
Ozone Oil Capsules ProtocolDose, timing and cautions for ozonated-oil capsules taken by mouth, with a plain note on the thin evidence.
Home
Ozone Oil Massage ProtocolHow much ozonated oil to use for muscle and joint massage, how often, and precautions, with the thin evidence stated plainly.
Home
Making Ozonated Oil Protocol (not advised)How manufacturers make ozonated oil, with the Madrid caution. MediSkill does not recommend making it at home.
6 protocols · At home · no generator needed.
A water-friendly alternative to ozone oil for nebulizing, drinking, skin, nose and mouth. Never for IV or injection.
No generator
Ozonated Glycerin ProtocolUses, mixing strengths, nebulizer set-up and precautions for ozonated glycerin, which needs no generator.
Home
Nebulizing Ozonated Glycerin ProtocolStrength, amount, mixing table and a step-by-step session for nebulizing diluted ozonated glycerin at home.
Home
Drinking Ozonated Glycerin ProtocolHow much ozonated glycerin to stir into a drink, how to build up over the first weeks, and what to watch for.
Home
Ozonated Glycerin for Skin and Beauty ProtocolMixing strengths, how often to use ozonated glycerin on the skin, and how to start gently.
Home
Ozonated Glycerin Nasal Spray ProtocolHow to mix ozonated glycerin with saline for a nasal pump spray, how much to spray, and how often.
Home
Ozonated Glycerin Mouthwash and Gum ProtocolHow to mix ozonated glycerin into a mouthwash, how long to swish, and how to use a small amount on the gums.
1 protocol · Home or clinic · water bubbled with ozone.
Water bubbled with ozone, used to drink, rinse or bathe. One sheet covers making it and using it.
Home or clinic
Ozone-Infused Water ProtocolConcentration, bubbling time, amounts and precautions for making and using ozone-infused water.
3 protocols · Skin conditions · home steps.
Acne and rosacea, eczema and psoriasis, and fungal infections, with the steps you can do at home.
Home
Acne and Rosacea Ozone ProtocolHome ozone steps for acne and rosacea: an ozonated water wash and ozonated oil, with what the studies used.
Home
Atopic Dermatitis and Psoriasis Ozone ProtocolHome ozone for atopic dermatitis and psoriasis: ozonated oil and ozone water, with what the studies used.
Home
Fungal Skin and Nail Infection Ozone ProtocolOil and water steps for ringworm, athlete's foot, candida and nail fungus, with the scalp spray and oil.
6 protocols · Licensed practitioners only · blood, IV and organ methods.
Blood, IV and organ methods for licensed practitioners: autohemotherapy, EBOO, and bladder instillation, plus the saline drip and 10-Pass methods we do not advise.
Clinic
IV Ozone Protocol: Major Autohemotherapy (MaH)Equipment, step-by-step directions, dosing and titration for IV ozone by major autohemotherapy (MaH).
Clinic
Minor Autohemotherapy (MiAH) ProtocolConcentration levels, steps, frequency and precautions for minor autohemotherapy (MiAH).
Practitioner
EBOO (cautioned)Madrid's ozone concentration, gas volume, blood flow and equipment notes for extracorporeal blood oxygenation-ozonation, an advanced hospital procedure. Standard IV ozone (MAH) comes first.
Clinic
Bladder (Vesicourethral) Ozone Instillation ProtocolPreparation, concentration and precautions for bladder irrigation with ozonized water, performed by a clinician.
Clinic
10-Pass Ozone Therapy ProtocolHow 10-Pass (high-dose multistep IV ozone) is commonly done, with the Madrid Declaration's position on it. MediSkill does not advise it.
Clinic
Ozonated Saline IV Drip (O3SS) ProtocolWeight-based dosing, preparation, concentration calculation and safety for the ozonated saline (O3SS) IV drip.
12 protocols · Licensed practitioners only · joint, spine and point injections.
Joint, spine, point and prostate injections for practitioners, with gas concentration, volume, sites and spacing for each.
Clinic
Knee Ozone Injection ProtocolLandmarks, injection angle, gas volume and schedule for ozone knee injections, with the Prolozone mixture and ozone-oxygen approaches side by side.
Clinic
Hip Ozone Injection ProtocolLateral approach, landmarks, needle and schedule for ozone hip injections, with the Prolozone mixture and ozone-oxygen approaches side by side.
Clinic
Shoulder Ozone Injection ProtocolPosterior landmarks, fluid volume and schedule for ozone shoulder injections, including a reported septic arthritis case, with Prolozone and ozone-oxygen approaches side by side.
Clinic
Elbow Ozone Injection ProtocolFluid and gas volumes and schedule for ozone elbow injections, with the Prolozone mixture and ozone-oxygen approaches side by side.
Clinic
Ankle Ozone Injection ProtocolSuperficial injection around the ankle's stabilizing structures, nerve cautions and schedule, with the Prolozone mixture and ozone-oxygen approaches side by side.
Clinic
Neck Ozone Injection ProtocolCervical landmarks, levels to avoid and the needle for ozone neck injections, with the Prolozone mixture and ozone-oxygen approaches side by side.
Clinic
Back Ozone Injection ProtocolLumbar landmarks, injection sites and volumes for paravertebral ozone back injections, with the Prolozone mixture and ozone-oxygen approaches side by side.
Practitioner · Injection
Trigger Point Ozone Injection ProtocolConcentration, volume per point, points per session and schedule for ozone trigger point injections, with Madrid and study doses side by side.
Practitioner · Injection
Acupuncture Point Ozone Injection ProtocolMicrodose volume, concentration and technique for ozone injected into acupuncture points, with Madrid and case series doses side by side.
Practitioner · Injection
Subcutaneous Ozone Injection ProtocolConcentration, volume per site, session limits and the glove technique for subcutaneous ozone injection, from Madrid and ISCO3.
Practitioner · Injection
PRP and Ozone Injection ProtocolOrder of injection, ozone concentration and volume, and PRP preparation from the studies that combined ozone with platelet-rich plasma.
Practitioner · Injection
Prostate Ozone Injection ProtocolMadrid's gas volume, concentration and weekly schedule for ozone injections in bacterial prostatitis and benign prostatic hyperplasia, with what small studies reported.
6 protocols · Dental professionals only · gas in the mouth and jaw.
Ozone gas, water and oil for decay, gums and implants, oral surgery, root canals, mouth sores and jaw osteonecrosis. Written for dental professionals.
Dentistry
Dental Ozone Protocol: Tooth Decay and SensitivitySettings published human studies used for gas on tooth decay, dentin hypersensitivity, bleaching sensitivity and white-spot prevention, by device family.
Dentistry
Dental Ozone Protocol: Gum Disease, Peri-Implantitis and Implant StabilitySettings published human studies used for gas in gum pockets, around inflamed implants and at implant placement. As an add-on to scaling, about half the trials found no added benefit.
Dentistry
Dental Ozone Protocol: Oral Surgery and HealingSettings published human studies used for gas after tooth extraction, at the implant drill site, on gum graft wounds and for orthodontic pain.
Dentistry
Dental Ozone Protocol: Root Canal Disinfection and Pulp CappingSettings published reports used for gas in root canals and on the exposed pulp. The evidence is case reports and one small controlled study.
Dentistry
Dental Ozone Protocol: Mouth Sores, Lichen Planus and UlcersSettings published human studies used for gas on aphthous ulcers, oral lichen planus and chemotherapy or post-extraction ulcers.
Dentistry
Dental Ozone Protocol: Medication-Related Osteonecrosis of the Jaw (MRONJ)Settings published reports used for gas in medication-related osteonecrosis of the jaw. Treatment reports are uncontrolled; the one prevention RCT found no difference.
3 protocols · Veterinary use · dogs, cats, horses and cattle.
Ozone for dogs, cats, horses and cattle, including common horse conditions. Doses scale with species and body weight.
Veterinary
Veterinary Ozone Protocol: Dogs and CatsRoutes, concentrations, volumes and schedules Madrid gives for ozone in dogs and cats: blood, rectal, injection, bagging, ozonated water and oil.
Veterinary
Veterinary Ozone Protocol: Horses and CattleSystemic and local routes Madrid gives for horses and cows: autohemotherapy, rectal and vaginal insufflation, joint and spine injections, bagging, ozonated water, oil and mastitis.
Veterinary
Veterinary Ozone Protocol: Horse ConditionsWhat Madrid lists for hooves, joints, tendons, back, skin, foals, wounds, reproduction and performance in horses, with concentrations, volumes and schedules.
In their own words
Lindsay O'Shea, APRN
Functional medicine clinic founder · attended a live event
“Not just the science behind ozone therapy… but also how do you take this and implement it into practice immediately.”
Dr. Akiba Green
Integrative wellness clinic, Lake Norman, North Carolina
“It’s the best decision I’ve done in the last six years.”
Next step
The Definitive Guide to Getting Started with Ozone Therapy: the methods, the equipment and staying safe.