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Ozonated Glycerin Nasal Spray Protocol
How to mix ozonated glycerin with saline for a nasal pump spray, how much to spray, and how often.
Quick answer: Mix ozonated glycerin to 10% in saline for a nasal pump spray, or start at 5% if you are sensitive. Use 1–2 sprays per nostril, 2–3 times a day.
Home method. Best done as recommended by a licensed provider who knows your health history.
What are the basics of ozonated glycerin nasal spray?
- A nasal spray is ozonated glycerin mixed with saline in a nasal pump bottle. It needs no generator or oxygen tank.
- Strength is 10%. If you are sensitive, start at 5%.
- Use 1 to 2 sprays per nostril, 2 to 3 times a day.
- Use saline only. Never use tap water in your nose.
- The amounts assume glycerin at about 10,000 ppm (parts per million, a strength label on the bottle).
Source: MediSkill's Ozone Protocol Guide for Clinics and published how-to guidance. Paraphrased.
How do you use ozonated glycerin as a nasal spray?
| Strength | How to mix | When to use it |
|---|---|---|
| 10% | 1 teaspoon ozonated glycerin added to 3 tablespoons of saline | Standard strength |
| 5% | 1/2 teaspoon ozonated glycerin added to 3 tablespoons of saline | If you are sensitive |
Spray 1 to 2 times per nostril, 2 to 3 times a day.
What are the best tips when using ozonated glycerin nasal spray?
- Glycerin is slow to blend with water. Swirl the mix in a clean glass cup until it looks even, then fill the bottle.
- Label the bottle with the strength and the date.
- Mix fresh. Keep leftovers in the fridge and use them within 24 hours.
- Store the bottle of undiluted ozonated glycerin away from heat and light. If you will not finish it within a few weeks, keep it in the fridge. Mixed batches keep in the fridge for up to 24 hours.
Is an ozonated glycerin nasal spray safe? Side effects and precautions
- Never use tap water in your nose.
- If the spray stings, drop to the 5% mix.
- Use it diluted in saline as described. Do not spray it undiluted.
- Never breathe ozone gas.
- Do not use ozonated glycerin during pregnancy. Check with your healthcare provider before starting, especially if you have a heart or bleeding condition or take blood thinners.
What to look for in a product: made with pure oxygen (not room air or a concentrator), lab-grade glycerin, a stated and tested concentration, and made fresh. The amounts on this sheet assume glycerin at about 10,000 ppm.
More detail
Equipment
- A clean nasal pump bottle.
- Sterile saline for the mix.
- A clean glass cup and a measuring spoon.
What the studies used
Study doses are listed as printed in each paper. They are not the amounts on this sheet.
- In a test-tube study, ozonated glycerol with 1,000 ppm or more inactivated 94% of SARS-CoV-2 within 20 seconds, and 99.8% after an hour (Takeda 2021). This was a lab study, not a nasal spray in people.
- An ozonized oil nasal spray (2 puffs, 100 µL in all, per nostril every 4 hours) was used together with oil capsules in an open-label series of 77 people. There was no control group, so it cannot show that the spray worked (Izzotti 2021).
Only 3 human studies of ozonated glycerin exist (one skin trial and two single-patient dental reports), and none was nebulized, swallowed, nasal or a mouthwash, so the studies below are on ozonated oils unless noted. No trial has tested an ozonated glycerin nasal spray.
Learn more
Background, benefits and research: Ozonized glycerin: benefits, uses and safety, full article. Related: Ozone therapy for ear, nose and throat conditions. Overview of all uses: Ozonated glycerin protocol. Comparing routes: Comparing home ozone therapies.
Related protocols: Ozonated Glycerin Mouthwash and Gum Protocol, Ozonated Glycerin Protocol, Nebulizing Ozonated Glycerin Protocol.
Other ozonated glycerin protocols
Educational reference only, not medical advice. Ozonated glycerin is not approved by the FDA for any medical use.
