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Practitioner protocol
Vaginal Ozone Insufflation Protocol
Dosing, schedule and precautions for vaginal ozone insufflation.
Quick answer: Vaginal ozone insufflation is a practitioner protocol that gives ozone gas through the vagina. It uses 17–40 µg/mL for 5–10 minutes at 1/8–1/4 LPM, 2–5 times a week.
Home method. Best done as recommended by a licensed provider who knows your health history.
What are the basics of vaginal ozone insufflation?
- Concentrations generally range from 17–40 ug/mL (gamma).
What dose and schedule are used for vaginal ozone insufflation?
| Weeks | Frequency | Amount | Flow rate | Strength |
|---|---|---|---|---|
| 1–2 | 2x per week | 5 minutes | 1/8–1/4 LPM | 17–34 gamma |
| 3–4 | 3x per week | 8 minutes | 1/8–1/4 LPM | 20–35 gamma |
| 5+ | 5x per week | 10 minutes | 1/8–1/4 LPM | 25–40 gamma |
The weeks column is how long to stay on that regimen. Start extremely slowly with these applications. It is a powerful modality. If you know you are sensitive, cut the starting points in half and slowly work your way up.
What equipment is needed for vaginal ozone insufflation?
- Ozone generator
- Oxygen tank
- Oxygen tank regulator (usually comes with the generator)
- Vaginal insufflation kit
If a regimen also includes rectal or IV ozone, they are typically not done on the same day.
What are the precautions for vaginal ozone insufflation, and who should avoid it?
- If there is negative feedback or discomfort, reduce time and frequency to a tolerable level.
- Vaginal insufflation can release more gas into the air than other methods. Don't breathe the gas: use a well-ventilated space and a fan if needed.
- There's no hard data comparing vaginal and rectal insufflation, but many believe it is similar: systemic, plus local to the reproductive system.
- Use a good-quality, body-safe lubricant if needed to prevent dryness, especially with daily use.
- Titration is critical to find what works and to gauge reactions.
- A "Herx" is possible. Consult a physician first; they may advise vitamin C, glutathione, activated charcoal and more. If it happens, stop for 4–7 days and restart at half the gamma, frequency and time.
- If there is cramping, bloating or gas, decrease the dose and frequency to a tolerable level.
- A Herx can show up as excessive fatigue, rash, and/or fever or chills.
Contraindications
Talk to your provider first. They will evaluate uncontrolled hyperthyroidism, G6PD deficiency, bleeding disorders and pregnancy.
Learn more
Background, benefits and research: Vaginal ozone insufflation, full article. Comparing routes: Comparing home ozone therapies.
Related protocols: Ozone Limb Bagging and Cupping Protocol, Breathing Ozonated Oil (BOO) Protocol, Ozone Sauna Protocol.
Other protocols
For licensed practitioners. Educational reference only, not medical advice. Follow your scope of practice and local regulations.

