How to Calculate a Dose of Ozone for Ozone Therapy
Ozone dose (µg) = concentration (µg/mL, also called gamma) × gas volume (mL)
Example: 200 mL of gas at 30 µg/mL = 6,000 µg (6 mg) of ozone.
Imagine a pot of coffee. Every morning there are two vital questions about this coffee. “How much is there?” and “How strong is it?”.
Dosing depends on your equipment. See our guide to ozone therapy machines for home.
There may be 12 cups of coffee in a full pot. This is the volume of coffee.
The coffee may be strong. This is the concentration of coffee.
In the same way, we measure ozone gas. “How much is there?” and “How strong is it?” These questions refer to the ozone concentration and volume. Once we understand both the concentration and volume, we can calculate the dose.
Ozone concentration
The concentration represents the strength of the ozone. It is commonly measured in ug/ml (micrograms per milliliter) and rarely in mg/l (milligrams per liter). We also use the word “gamma” to describe the concentration of ozone.
Volume of gas
The volume of gas is represented by the quantity of gas. It is usually measured in cubic centimeters (CC) or milliliters (ml).
This picture shows different concentrations but the same volume.
Calculating the dose
A dose of ozone is often measured in micrograms (ug). So we say things like, “He dosed the patient with 3,000 micrograms of ozone.”
Calculating a dose of ozone is extremely easy. You multiply the concentration by the volume.
(concentration in ug/ml) x (volume in ml) = (dose in ug)
So if we have a 10cc syringe with 30 gamma the following formula is applied:
(30ug/ml) x (10ml) = 300ug
You must know BOTH the concentration and volume to understand the dose. Then multiply them together.
What is gamma?
“Gamma” is shorthand for micrograms per milliliter. 1 gamma = 1 µg/mL = 1 mg/L, so a generator chart that says 30 mg/L means the same strength as 30 gamma.
Doses are usually written in micrograms (µg). 1,000 µg = 1 mg.
Ozone dose chart
Find the concentration on the left and the volume across the top. The number where they meet is the dose.
| Concentration | 10 mL | 50 mL | 100 mL | 200 mL |
|---|---|---|---|---|
| 10 µg/mL | 100 µg | 500 µg | 1,000 µg | 2,000 µg |
| 20 µg/mL | 200 µg | 1,000 µg | 2,000 µg | 4,000 µg |
| 30 µg/mL | 300 µg | 1,500 µg | 3,000 µg | 6,000 µg |
| 40 µg/mL | 400 µg | 2,000 µg | 4,000 µg | 8,000 µg |
| 50 µg/mL | 500 µg | 2,500 µg | 5,000 µg | 10,000 µg |
| 60 µg/mL | 600 µg | 3,000 µg | 6,000 µg | 12,000 µg |
| 70 µg/mL | 700 µg | 3,500 µg | 7,000 µg | 14,000 µg |
Worked examples
- Rectal insufflation: 200 mL at 20 µg/mL = 4,000 µg. Our clinic protocol uses 200 to 400 mL at 10 to 40 µg/mL (40 is the maximum), so a session is roughly 2,000 to 16,000 µg; most people start at 200 mL. See the rectal ozone protocol.
- IV ozone (major autohemotherapy): 200 mL of gas at a starting 30 µg/mL = 6,000 µg; at the 70 µg/mL maximum it is 14,000 µg. See the IV ozone protocol.
- Ear insufflation and limb bagging: the gas flows continuously, so these methods are set by concentration and time rather than a total dose. See the ear and limb bagging protocols.
Concentration depends on your generator and oxygen flow
You don’t measure concentration yourself. Your generator’s chart tells you the µg/mL it produces at each setting and oxygen flow rate, and on most home units a slower flow makes stronger gas. That’s why the chart has to match your generator and regulator. See how to choose an ozone machine and oxygen for ozone therapy.
Practitioners: dosing and titration for IV ozone are covered step by step in our IV Ozone 101 training.
Frequently asked questions
What is an ozone dose?
An ozone dose refers to the total amount of ozone delivered, usually measured in micrograms (µg). It is determined by combining the ozone concentration with the volume of gas administered.
How is ozone concentration measured?
Ozone concentration is typically measured in micrograms per milliliter (µg/mL), also called gamma. It represents how strong the ozone gas mixture is.
How is the volume of ozone gas measured?
The volume of ozone gas is measured in milliliters (mL) or cubic centimeters (cc) and represents how much gas is administered.
How do you calculate an ozone dose?
To calculate an ozone dose, multiply the concentration by the volume: (concentration in µg/mL) × (volume in mL) = dose in micrograms (µg).
Why is it important to calculate ozone dose accurately?
Accurate ozone dosing ensures safety and effectiveness. Too little ozone may be ineffective, while too much can cause irritation or unwanted oxidative stress.
Methods, equipment and safety basics in one place. We send it to your inbox.
Check your inbox: your free guide is on its way.
Something went wrong. Please try again.
