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Nebulizing Ozonated Glycerin Protocol
Strength, amount, mixing table and a step-by-step session for nebulizing diluted ozonated glycerin with a plug-in compressor nebulizer.
Quick answer: To nebulize ozonated glycerin, dilute it and run about 5 mL through a plug-in compressor nebulizer for 10–15 minutes. Start at 2.5% (1% if sensitive), 1–2 times a day, with a maximum of a 10% mix and 5 mL twice a day.
Home method. Best done as recommended by a licensed provider who knows your health history.
What are the basics of nebulizing ozonated glycerin?
- Nebulizing turns diluted ozonated glycerin into a fine mist you breathe in through a face mask. It is a home method and needs no ozone generator or oxygen tank.
- Never breathe ozone gas. When you nebulize ozonated glycerin, you breathe a fine mist of diluted glycerin and water. No ozone gas from a generator goes into the mask (see why you can't breathe ozone).
- Strength is the percent of ozonated glycerin in the total mix. Start light and increase only if it feels comfortable: 1% is extra gentle, 2.5% is where to start, 5% is the step up, and 10% is the upper limit.
- Move up one step after a few comfortable sessions. If a session feels heavy, go back to the previous strength.
- One session is about 5 mL of mix, run until the cup is empty (10–15 minutes), once or twice a day.
- The amounts follow published practitioner guidance. Nebulizing ozonated glycerin itself has not been tested in a clinical trial.
Source: MediSkill's Ozone Protocol Guide for Clinics; practitioner guidance reported in Rowen RJ, The Rowen Report. Paraphrased.
How do you nebulize ozonated glycerin?
Most nebulizer cups hold 5 to 8 mL. Mix a 5 mL batch for each session, or a 10 mL batch if you want easier measuring at the low strengths. Use distilled water.
| Strength | For a 5 mL cup | For a 10 mL batch | Where it fits |
|---|---|---|---|
| 1% | 0.05 mL glycerin + 4.95 mL water | 0.1 mL glycerin + 9.9 mL water | Extra gentle; for sensitive people |
| 2% | 0.1 mL glycerin + 4.9 mL water | 0.2 mL glycerin + 9.8 mL water | Between 1% and 2.5% |
| 2.5% | 0.125 mL glycerin + 4.875 mL water | 0.25 mL glycerin + 9.75 mL water | Start here |
| 5% | 0.25 mL glycerin + 4.75 mL water | 0.5 mL glycerin + 9.5 mL water | Step up |
| 10% | 0.5 mL glycerin + 4.5 mL water | 1.0 mL glycerin + 9.0 mL water | Upper limit |
Measuring 0.05 mL is fiddly. For 1%, mix the 10 mL batch, load 5 mL into the cup, and keep the rest in the fridge for your next session within 24 hours. With spoons instead of syringes: 3 tablespoons of water plus 1/4 teaspoon of glycerin is 2.5%, and 1/2 teaspoon is 5%. That makes about 45 mL, so pour about 5 mL into the cup and keep the rest in the fridge for up to 24 hours.
A session, step by step
- Measure the ozonated glycerin with the 1 mL syringe and put it in the glass cup.
- Measure the water with the 10 mL syringe and add it.
- Swirl for 2 to 3 minutes. Glycerin doesn't want to mix with water, so keep swirling until the liquid looks even.
- Pour it into the nebulizer cup and attach the cup to the mask.
- Connect the tube to the compressor and turn it on. You should see mist within a few seconds.
- Put on the mask and breathe normally, sitting upright and relaxed. Don't force deep breaths; breathe in gently through the nose.
- Run until the cup is empty, about 10 to 15 minutes.
- Rinse the cup and mask with warm water and let them air dry. Replace the cup on the maker's schedule, since glycerin residue builds up.
What are the best tips when using nebulizing ozonated glycerin?
- Keep one syringe for the glycerin and one for the water, and label them so they don't get swapped.
- Glycerin is thick. Draw it slowly and wipe the syringe tip before dispensing.
- Distilled water is preferred because it is easy to find. Saline also works.
- Plug-in units make a denser mist than battery handhelds.
- Many nebulizer kits ship with only a mouthpiece, so check that an adult face mask is in the box.
- Store the bottle of undiluted ozonated glycerin away from heat and light. If you will not finish it within a few weeks, keep it in the fridge. Mixed batches keep in the fridge for up to 24 hours.
Is nebulized ozonated glycerin safe? Side effects and precautions
- Never nebulize undiluted ozonated glycerin. Always mix it with water first.
- If the mist feels heavy, or you cough or feel chest tightness, stop and use a weaker mix next time. Stop if it irritates your airways.
- Never use tap water in the nebulizer.
- Do not use a mesh or ultrasonic nebulizer. Glycerin clogs the mesh, and ultrasonic units are unreliable with it.
- Follow your nebulizer maker's instructions for use and cleaning.
- Never breathe ozone gas.
- Talk to your provider first. They will evaluate uncontrolled hyperthyroidism, G6PD deficiency, bleeding disorders and pregnancy.
What to look for in a product: made with pure oxygen (not room air or a concentrator), lab-grade glycerin, a stated and tested concentration, and made fresh. The amounts on this sheet assume glycerin at about 10,000 ppm (parts per million, a strength label on the bottle).
More detail
Equipment
- A plug-in compressor (jet) nebulizer with an adult face mask. This is the standard tabletop kind with a small air pump, a tube, a liquid cup and a mask. Most nebulizer cups hold 5 to 8 mL.
- A 1 mL oral syringe with no needle, marked in 0.01 mL, for the glycerin.
- A 10 mL syringe with no needle, for the water.
- A small glass cup or shot glass for mixing (glass, not plastic).
- Distilled water (saline also works).
What the studies used
Study doses are listed as printed in each paper. They are not the amounts on this sheet.
- Practitioner guidance (not a trial): Dr. Robert Rowen's clinic guidelines start at 1% to 2.5% and allow up to 5 mL twice a day. He has also published case reports of people nebulizing it daily at 5% to 10% (Rowen RJ, The Rowen Report).
- No study has tested nebulizing ozonated glycerin, and no study supports breathing ozone gas. Madrid lists inhaling ozone gas as a route that should never be used.
Only 3 human studies of ozonated glycerin exist (one skin trial and two single-patient dental reports), and none was nebulized, swallowed, nasal or a mouthwash.
Learn more
Background, benefits and research: Ozonized glycerin: benefits, uses and safety, full article. Why gas is different: Why you can't breathe ozone. Overview of all uses: Ozonated glycerin protocol. Comparing routes: Comparing home ozone therapies.
Related protocols: Drinking Ozonated Glycerin Protocol, Ozonated Glycerin for Skin and Beauty Protocol, Ozonated Glycerin Nasal Spray Protocol.
Other ozonated glycerin protocols
Educational reference only, not medical advice. Ozonated glycerin is not approved by the FDA for any medical use.
