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Practitioner protocol
Ozone-Infused Water Protocol
Concentration, bubbling time, amounts and precautions for making and using ozone-infused water.
Quick answer: Ozone-infused water is made by bubbling ozone through water. The sheet uses 38–70 µg/mL for 12 minutes per 8 oz, with 4–16 oz used 2–14 times a week. It is a practitioner protocol.
Home method. Best done as recommended by a licensed provider who knows your health history.
What are the basics of ozone-infused water?
- Concentration ranges from 38–70 ug/mL (gamma).
- Used for drinking, oral swishing, or cleansing an affected area.
- It takes 24 minutes to fully saturate a 16 oz flask of water.
What dose and schedule are used for ozone-infused water?
| Use | Frequency | Amount | Time to bubble | Strength |
|---|---|---|---|---|
| Drinking water | 2–14x per week | 4–16 oz | 12 minutes per 8 oz at 1/4 LPM | 38–70 gamma |
Start extremely slowly with these applications. It is a powerful modality. If you know you are sensitive, cut the starting points in half and slowly work your way up.
What are the best tips when using ozone-infused water?
- Ozonated water has a very short half-life; drink it right after making it.
- There is no hard data on how much is too much; it's not advised to exceed 16 oz a day.
- Don't use a plastic cup; the gas can react with some materials. Glass or stainless steel is best.
- Some like it stronger, others weaker; find what works.
What equipment is needed for ozone-infused water, and what else can it do?
Equipment
- Oxygen tank
- Oxygen regulator (usually comes with the generator)
- Ozone generator
- Water bubbling device
Mouth (dental) use
- Daily swishing is only recommended when there is an oral infection or issue.
- Swish 8 ounces in the mouth for 60 seconds, up to twice a day, until the issue resolves.
- For infections deep in a tooth, oil pulling with ozone oil is generally preferred: swish 15–20 minutes, twice a day.
Washing wounds
- Limb bagging and ozone oil are generally preferred for wounds, ulcers and infections.
- Pour ozonated water over the area, and into the wound if possible, 2–3 times a day until resolved.
What are the precautions for ozone-infused water, and who should avoid it?
- If there's negative feedback after drinking, halve the gamma and time next time, or stop.
- Some people get headaches or a scratchy throat after drinking it; halve the gamma and time next time, or stop.
- Stop if you get irritation or discomfort.
- Pregnancy: check with your healthcare provider before starting.
Learn more
Background, benefits and research: Ozone water, full article. Comparing routes: Comparing home ozone therapies.
Related protocols: Fungal Skin and Nail Infection Ozone Protocol.
Other protocols
For licensed practitioners. Educational reference only, not medical advice. Follow your scope of practice and local regulations.

