Knowledge Center › Protocols › Mouthwash and gums

Home protocol

Ozonated Glycerin Mouthwash and Gum Protocol

How to mix ozonated glycerin into a mouthwash, how long to swish, and how to use a small amount on the gums.

What are the basics of ozonated glycerin mouthwash?

• Ozonated glycerin is water-soluble, so it rinses clean without the oily film of ozonated oil.
• Mouthwash: mix it at 10% in water, swish for 1 to 2 minutes and spit. A drop of peppermint oil is optional.
• Gums: massage a pea-sized amount of full-strength glycerin into the gum line before bed.
• Use the mouthwash 1 to 2 times a day.
• The amounts assume glycerin at about 10,000 ppm (parts per million, a strength label on the bottle).

Source: MediSkill's Ozone Protocol Guide for Clinics and published how-to guidance. Paraphrased.

How do you use ozonated glycerin as a mouthwash?

What are the best tips when using ozonated glycerin mouthwash?

• Rinse, then spit. Do not swallow the mouthwash.
• Add a drop of peppermint oil to the mouthwash if you like the taste.
• Mix the mouthwash fresh. Keep leftovers in the fridge and use them within 24 hours.
• Store the bottle of undiluted ozonated glycerin away from heat and light. If you will not finish it within a few weeks, keep it in the fridge. Mixed batches keep in the fridge for up to 24 hours.

Is ozonated glycerin mouthwash safe? Side effects and precautions

• Ozonated glycerin is slightly acidic. Strong mixes can irritate the lining of the mouth. If it stings, dilute the mouthwash further or use less.
• Use only a pea-sized amount at full strength on the gums.
• This is not a replacement for dental care. See your dentist for gum or tooth problems.
• Never breathe ozone gas.
• Do not use ozonated glycerin during pregnancy. Check with your healthcare provider before starting, especially if you have a heart or bleeding condition or take blood thinners.

More detail

Equipment

• A clean cup and a measuring spoon. Nothing else is needed.

What the studies used

Study doses are listed as printed in each paper. They are not the amounts on this sheet.

• The studies of ozone in the mouth used ozonated olive oil. In 96 adults with gum disease, a 30-second rinse with 12 mL of ozonated olive oil mouthwash three times a day for 3 months lowered salivary MMP-8 (a gum inflammation marker) more than cleaning alone. Allocation was by birth date and there was no placebo (Nardi 2020).
• In 63 adults with early gum disease after cleaning, ozonated olive oil toothpaste plus mouthwash twice daily for 30 days lowered plaque by 33% and placebo by 24%, which was not a significant difference. Pocket depth fell more with the ozonated oil (0.4 vs 0.1 mm) (Abbinante 2026).
• In 30 people with recurring mouth ulcers, 2 drops of ozonated oil 4 times a day for 5 days brought pain down to 1.5 on a 10-point scale, against 1.8 with plain sesame oil and 4.6 with a water placebo (Dharmavaram 2015).

Only 3 human studies of ozonated glycerin exist (one skin trial and two single-patient dental reports), and none was nebulized, swallowed, nasal or a mouthwash, so the studies below are on ozonated oils unless noted. No trial has tested an ozonated glycerin mouthwash.

Learn more

Background, benefits and research: Ozonized glycerin: benefits, uses and safety, full article. Dental care with ozone: Ozone in dentistry. Overview of all uses: Ozonated glycerin protocol. Comparing routes: Comparing home ozone therapies.

Related protocols: Ozonated Glycerin Protocol, Nebulizing Ozonated Glycerin Protocol, Drinking Ozonated Glycerin Protocol.

Other ozonated glycerin protocols

Educational reference only, not medical advice. Ozonated glycerin is not approved by the FDA for any medical use.