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Practitioner protocol
Breathing Ozonated Oil (BOO) Protocol
Concentration, flow, schedule and precautions for breathing the vapor from ozone bubbled through olive oil.
Quick answer: Breathing ozonated oil (BOO) means breathing the vapor from ozone bubbled through olive oil. The sheet uses 10–30 µg/mL at a flow of 1/2 LPM for 15–30 minutes, 3–5 times a week. It is a practitioner protocol.
Home method. Best done as recommended by a licensed provider who knows your health history.
Do NOT breathe this gas directly. It must first be bubbled through olive oil.
What are the basics of breathing ozonated oil (BOO)?
- Concentration ranges from 10–30 ug/mL (gamma).
- Gas flow at 1/2 LPM with the generator around setting 4. Flow through the flask matters.
- Use about 8–10 ounces of olive oil.
What dose and schedule are used for breathing ozonated oil (BOO)?
| Weeks | Frequency | Amount | Flow rate | Strength |
|---|---|---|---|---|
| 1 | 3x per week | 15 minutes | 1/2 LPM | 13–25 gamma (setting 4) |
| 2+ | 5x per week | 20–30 minutes | 1/2 LPM | 13–25 gamma (setting 4) |
The weeks column is how long to stay on that regimen. Start extremely slowly with these applications. It is a powerful modality. If you know you are sensitive, cut the starting points in half and slowly work your way up.
What are the best tips when using breathing ozonated oil (BOO)?
- Best done with olive oil as the base.
- A person can reuse their own cannula; wipe it with an alcohol wipe after use.
What equipment is needed for breathing ozonated oil (BOO)?
- Oxygen tank
- Oxygen regulator (usually comes with the generator)
- Ozone generator
- Oil bubbling device
- Nasal cannula
What are the precautions for breathing ozonated oil (BOO), and who should avoid it?
- Decrease the time as needed. Start at half the guidelines if helpful.
- The oil can be reused because it doesn't touch the person; change it after every 2–4 hours of use.
- Some people get headaches after a session; cut the time or the gamma in half if that happens.
Contraindications
Talk to your provider first. They will evaluate uncontrolled hyperthyroidism, G6PD deficiency, bleeding disorders and pregnancy.
Learn more
Background, benefits and research: Breathing ozonated oil (BOO), full article. Comparing routes: Comparing home ozone therapies.
Related protocols: Ozone Sauna Protocol, Rectal Ozone Insufflation Protocol, Ozone Ear Insufflation Protocol.
Other protocols
For licensed practitioners. Educational reference only, not medical advice. Follow your scope of practice and local regulations.

